INDUCTION OF FRESH CHOCOLATE CYST SLURRY INTO ENDOMETRIOSIS MICE MODEL Ivanna Beru Brahmana (a,b*) - Soetrisno(b)- Mulyoto Pangestu (c) -Rita Cempaka (d)- Ika Puspitasari (e)
a. Obstetrics and Gynecology Departement, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Bantul, Indonesia, 55183
ivanna.beru.brahmana[at]gmail.com*
b. Doctoral Program of Medical Science, Universitas Sebelas Maret, Surakarta, Indonesia, 57126
c. Education Program in Reproduction and Development, Dept. Obstetrics and Gynaecology, Monash University, Australiad.
d. Departement of Pathology Anatomy, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281
e. Faculty of Pharmacy / Academic Hospital, Universitas Gadjah Mada, Yogyakarta, Indonesia, 55281
Abstract
Background: The Covid-19 pandemic has prevented laparoscopy or laparostomy surgery, except in emergency. The endometrial lesions from laparoscopy or laparotomy to induce endometriosis-associated mice is not available. Chocolate cyst is one type of endometriosis in addition to the appearance of endometrial lesions. The limitations of obtaining these samples led to the idea of trying to induce fresh chocolate cyst pulp to become a model of endometriosis mice. Chocolate cyst slurry can be obtained through cyst puncture, no need to perform laparoscopy or laparotomy. Objective: to prove that induced mice with fresh chocolate cyst pulp samples succeeded in becoming endometriosis models of mice. Methods: Fresh chocolate cyst slurry was injected as much as 5.10-3 ml.g-1 of mice weight intraperitoneally on day zero. Mice of Mus musculus strain Balb/c were injected with cyclosporine 1.4.10-2 mg.g-1 of weight of mice intraperitoneally dayly for 14 days. On the 1st and 5th day the mice were injected with estradiol 2.6.10-4 ml.g-1 of the weight of the mice intramuscularly. Induction research on endometriosis model mice was conducted at the Pharmacology Laboratory of the Gadjah Mada University, Yogyakarta, Indonesia. On the 15th day the mice were necropsied for histopathological examination at the Anatomical Pathology Laboratory, Gadjah Mada University, Yogyakarta, Indonesia with Hematoxylin-Eosin (HE) staining. Results and conclusions: A total of 6 mice that were induced with fresh chocolate cyst pulp histopathologically showed signs of endometriosis. All of them were 100% successful in becoming endometriosis model mice, as a novelty in endometriosis studies using experimental animals.
Keywords: fresh chocolate cyst pulp-endometriosis- endometriosis model mice- anatomical pathology
Topic: Medical : basic science, clinical, translational research, medical education, and miscellaneous
