Mesenchymal Stem Cell Secretome Decrease Inflammation in Sepsis Model Arifin1,2), Bambang Purwanto1,2), Dono Indarto1,3), Brian Wasita1,4), Tatar Sumanjar1,2), Eti Poncorini1,5), Soetrisno1,6)
1 Doctoral Program of Medical Sciences, Faculty of Medicine, Universitas Sebelas Maret, Jl. Ir Sutami No 36A, Kentingan, Jebres, Surakarta, 57126
2 Department of Internal Medicine, Faculty of Medicine, Universitas Sebelas Maret, Jl. Ir Sutami No 36A, Kentingan, Jebres, Surakarta, 57126
3 Department of Physiology, Faculty of Medicine, Universitas Sebelas Maret, Jl. Ir Sutami No 36A, Kentingan, Jebres, Surakarta, 57126
4 Department of Anatomic Pathology, Faculty of Medicine, Universitas Sebelas Maret, Jl. Ir Sutami No 36A, Kentingan, Jebres, Surakarta, 57126
5 Department of Public Health, Faculty of Medicine, Universitas Sebelas Maret, Jl. Ir Sutami No 36A, Kentingan, Jebres, Surakarta, 57126
6 Department of Obstetrics and Gynaecology, Faculty of Medicine, Universitas Sebelas Maret, Jl. Ir Sutami No 36A, Kentingan, Jebres, Surakarta, 57126
Abstract
Inflammation plays an essential role in sepsis by activating Nf-Kβ- (nuclear factor-kappa Beta). Mesenchymal stem cell secretome (MSC) is one of the breakthroughs in sepsis. The secretion of mesenchymal stem cells consists of cytokines, microRNA (miRNA), exosomes, and microvesicles, which can cause a therapeutic effect. The mechanisms of MSC secretion include antiapoptotic, anti-inflammatory, antifibrotic, angiogenic, and the development of tissue regeneration. This study aims to determine the impact of MSC secretomes on Nf-Kβ- expression in the liver and kidney. This research is a posttest-only control design group experimental study. This experiment used 40 rats. There were eight mice per group, and the animals were randomized, the control group, the sepsis model group, the sepsis model group receiving MSC Secretome 150 l/20 gr, the sepsis model group receiving MSC Secretome 300 l/20 gr, and the sepsis model group receiving secretome. MSC 600 l/20 gr. After four days, they were killed, and their liver and kidneys were removed for examination. Data were analyzed using the Shapiro-Wilk, Anova, and Post-Hoc tests. Significance using p<0.05. MSC secretion decreases Nf-Kẞ- expression in the liver and kidney. MSC secretome 600 l / 20 gr is the best dose for fighting sepsis models. MSC secretion decreased Nf-Kẞ- expression in a mouse model of liver and kidney sepsis.
