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Abstract
ABS-433
Optimization of an Ointment Formula Combination of Ethanol Leaf Extract (Muntingia calabura L.) and Manuka Honey for Burns
Annisa Nuraini Oktavia (a*)- Ratna Wijayatri (b)- Eka Sakti Wahyuningtyas (b)- Alfian Syarifuddin (b)
Corresponding Author: Annisa Nuraini Oktavia


Question from dr. Mirah Rejeki
2022.12.21 14:53:17

It is a interesting paper. How do you make burns in experimental animals?

Reply from Ms. Ratna Wijayatri
2022.12.21 15:45:55

thank you for your question. we make burn in experimental animal with Making minor burn wounds on the skin of mice was carried out using a tool made of circular conductors with a diameter of 4 mm. Initially, the mice were anesthetized using a liquid combination of Ketamine and Xylazine with the intramuscular route of administration after that A tool made of a conductor is heated over a fire for 30 seconds and then attached for a few moments to both sides of the mice to form a burn by taking the dermis of the mice. i hope this answer can explain.

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