THE EFFECTIVENESS OF A COMBINATION of Piper betle L. AND MANUKA HONEY EXTRACT GEL SPRAY TO ACCELERATING ACUTE WOUND HEALING Ratna Wijayatri, Eka Sakti Wahyuningtyas, Alfian Syarifuddin
Universitas Muhammadiyah Magelang
Abstract
Background: Acute wounds are tissue injuries that are at risk of infection, therefore acute wound care must be considered carefully. Piper betle leaf contains flavonoids that play a role in the wound healing process, Manuka honey also contains vitamins that can support the phase of wound healing. The combination of Piper betle L. and manuka honey is made into a spray gel preparation because it has advantages over other topical preparations. Objective: This study aims to test the effectiveness of wound healing spray gel combination of Piper betle L. and Manuka honey on the acute wound of mice. Methods: This study uses experimental research with a randomized matched post-test only control group design. Results: The formulation taken were maceration extraction from Piper betle L. Simplicia powder with 70% ethanol then evaporated to obtain a thick extract. Spray gel preparations of a combination of Piper betle L extract and manuka honey were made with a concentration of 1% Piper betle L and Manuka honey, 3% Piper betle L and Manuka honey, and 5% Piper betle L and Manuka honey. Balb/c mice that had full-thickness acute wounds were divided into 7 treatment groups, namely SM1, SM2, SM3, M, KP, B, D. Mice were treated for 14 days and the wound area ratio was calculated and analyzed by ANOVA. The test results showed a significant difference between groups SM3-M, SM5-M, M-SM3, M-SM5, KP-M, B-SM3, BM with a significance value below 0.05 which proves that the spray gel combination of Piper betle L extract and manuka honey, manuka honey, oxoferin, and base both have activity in accelerating acute wound healing.
