The Effect of Indian Sandalwood Essential Oil (Santalum Album Linn) in Inhibiting The Growth of Biofilm Streptococcus Mutans ATCC 25175 Please Just Try to Submit This Sample Abstract Ciptadhi Tri Oka Binartha, Velicia Ghina Nadhifa
Faculty of Dentistry, Universitas Trisakti Jl. Kyai Tapa no 260 Jakarta Barat, Indonesia
Abstract
One of the main causes of dental caries is Streptococcus mutans. Chlorhexidine gluconate 0.2% it is a gold standard for antibacterial mouthwash. Chlorhexidine gluconate can cause an allergic reaction and produce staining on teeth if use in the long term. Indian sandalwood essential oil is a natural substance that has an antibacterial properties with less side effects.
To determine the effect of Indian sandalwood essential oil (Santalum album Linn) extract in inhibiting the growth of Streptococcus mutans ATCC 25175 biofilm.
Streptococcus mutans ATCC 25175 was cultured in nutrient broth and incubated at 37 C for 24 hours. Biofilm was made by inoculating the culture to a 96 well plate. Well plate then incubated for 24 hours. Indian sandalwood essential oil with concentration of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.56% was made. Biofilm was tested by using the biofilm assay method with 15 and 30 minutes time. Chlorhexidine gluconate 0.2% was used as positive controls. The result was obtained using a microplate reader by observing the optical density values at 595 nm wavelength.
Indian sandalwood essential oil with the best concentration is 1.56% because can inhibit the growth of Streptococcus mutans ATCC 25175 biofilm. ANOVA test shows that there are significant differences in each concentration groups compared to 0.2% chlorhexidine gluconate.
Indian sandalwood essential oil (Santalum album Linn) can inhibit the growth of Streptococcus mutans ATCC 25175 biofilm.
Keywords: Indian sandalwood essential oil (Santalum album Linn), biofilm, Streptococcus mutans, dental cariesPlease Just Try to Submit This Sample Abstract
