THE EFFECTIVE DOSE OF POMEGRANATE PEEL EXCTRACT TO IMPROVE SGPT ENZYME AND PLASMA MDA LEVELS IN RATS WHICH INDUCED BY CCL4 Fransiska Ambarukmi Pontjosudargo
FK Unjani
Abstract
The study was conducted to determine the effective dose of pomegranate peel extract against liver damage by measuring SGPT and MDA plasma levels in male rats induced by CCl4. The target of this study is to prove the inhibitory effect of pomegranate peel extract on rat liver damage due to CCl4 induction and to obtain the optimal dose. This was a laboratory study with a completely randomized design, using a sample of 36 rats with the number of replications per group was 4 rats. The objects are grouped into 9 groups which will compare the effects of pomegranate peel extract in various doses ranging from 100 mg/KgBW to 600 mg/KgBB. Indicators of inhibition of liver damage in terms of plasma SGPT and MDA levels. Data analysis used the Kruskal Wallis test followed by the Mann-Whitney test. The results showed that the treatment group with a dose of 300 mg/KgBW to 600 mg/KgBW had significantly lower plasma levels of SGPT and MDA (P <0.05) compared to the positive control who was given CCl4. Pomegranate peel extract at a dose of 500 mg/KbBW was the most effective at inhibiting the increase in SGPT levels, while the doses of 400 mg/KgBW and 500 mg/KgBW were able to inhibit the increase in plasma MDA levels with the same effectiveness (p>0.05). The ethanol extract of pomegranate peel was able to inhibit the increase in SGPT levels with the most effective dose of 500 mg/KbBW and inhibit plasma MDA levels with the most effective dose of 400 mg/KgBW.
