The Effect of pH on The Activity Of Bacteriocin From Lactobacillus acidophilus On The Growth Of Salmonella typhi Elizabeth Noviana Lumban Raja (a), Siska Telly Pratiwi (b*), Alexander Siagian (c)
a) Medicine Study Program, Faculty of Medicine, Universitas Jenderal Achmad Yani, Jenderal Sudirman Street, Cibeber, South Cimahi, Cimahi City, West Java, Indonesia 40531
b) Department of Microbiology, Faculty of Medicine, Universitas Jenderal Achmad Yani, Jenderal Sudirman Street, Cibeber, South Cimahi, Cimahi City, West Java, Indonesia 40531
*siska.telly[at]lecture.unjani.ac.id
c) Department of Anesthesiology and Intensive Therapy, Faculty of Medicine, Universitas Jenderal Achmad Yani, Jenderal Sudirman Street, Cibeber, South Cimahi, Cimahi City, West Java, Indonesia 40531
Abstract
Typhoid fever is one of the most serious infectious diseases in Indonesia. Antibiotic resistance is one of the problems associated with the treatment of typhoid fever. L.acidophilus produces antimicrobial substances, one of which is bacteriocin. Bacteriocin has bactericidal and bacteriostatic activity. The aim of this study was to determined the activity of bacteriocin from L. acidophilus against Salmonella typhi in vitro as antimicroba. The design of this study was laboratory experimental with post test only control group design using the Kirby-Bauer agar diffusion method on Mueller Hinton Agar (MHA). The bacteriat of this study were S. typhi ATCC 6539 and bacteriocin from L. acidophilus ATCC 4356 in various pH (2, 4, 6, 8, 10, and 12). The result of observation was carried out by measuring the inhibition zone in each treatment. The result showed bacteriocin from L. acidophilus at pH 2 had an inhibition zone 4.91 plus minus 1.70 mm. Statistical calculation using the One Way Anova test showed hat p-value was 0.000 then continued with the Post Hoc Tukey test showed bacteriocin from L. acidophilus at pH 2 inhibited the growth of S. typhi very significant compared to kloramfenikol. The conclusion of this study is that bacteriocin from L. acidophilus can be extracted. L. acidophilus can inhibit the growth of S. typhi and has an optimal pH, which is at pH 2 with a weak category of inhibitory strength. The bacteriocin L. acidophilus ATCC 4356 could not compete with chloramphenicol as the first line drug for typhoid fever.
