EFFECT OF TURMERIC RHIZOME ETHANOL EXTRACT (Curcuma Longa) ON BLOOD HEMOSTASIS TEST Rini Roslaeni(a*), Raya Agung Maha Sakti(b), M Fathan Zulfahmi(b), Maharany Rizky Yuniar(b), Evi Sovia(c), Wahyu Harihardjaja(d), Anita Liliana Susanti(a)
aClinical Pathology Laboratory Fakultas Kedokteran Universitas Jenderal Achmad Yani Cimahi
*rini.roslaeni[at]lecture.unjani.ac.id
bMedical Study Program Fakultas Kedokteran Universitas Jenderal Achmad Yani Cimahi
cPharmacology Laboratory Fakultas Kedokteran Universitas Jenderal Achmad Yani Cimahi
dInternal Medicine Laboratory Fakultas Kedokteran Universitas Jenderal Achmad Yani Cimahi
Abstract
Turmeric (Curcuma longa) is a medicinal plant that is widely found in Asia, often used as spice or traditional medicine. Turmeric plant has dark yellow roots (rhizome) and contains various important compounds, including curcumin and ar-tumeron which play a role in inhibiting blood clotting. Currently, one of the main causes of morbidity and mortality in the world is cardiovascular disease such as stroke and coronary heart disease. The potential of turmeric which has anticoagulant properties is expected to be an alternative in prevention of cardiovascular disease. The purpose of this study was to determine the effect of ethanol extract of turmeric rhizome in vitro and in vivo on the prothombine time (PT), bleeding time (BT), and clot retraction. Subjects of in vitro study were men aged 17-20 years. History of platelet aggregation abnormalities, hemophilia, anemia and thrombocytopenia were excluded. In vivo research subjects used male mice aged 1-2 months, weighing 20-30 grams and healthy. The PT examination used tilt tube method, clot retraction examination used whole blood then left for 2 hours and the remaining serum was calculated using formula for serum volume/initial blood volume x100%, while BT examination was carried out by incising rats tail. Results showed that mean PT and BT were elongated in all test groups. The greater the dose of turmeric rhizome extract, the longer the PT and BT values. Clot retraction in the test group showed a lower mean serum percentage compared to negative controls, and the greater the extract dose given, the lower the clot retraction value. This is suspected because the active substances in turmeric rhizome extract such as curcumin and ar-tumeron can inhibit the blood clotting so that the blood hemostasis tests affected
Keywords: bleeding time, curcumin, clot retraction, prothrombine time, turmeric
