ACUTE TOXICITY TEST OF ETHANOL EXTRACT RED CURLY CHILI FRUIT (Capsicum annum L) IN MICE (Mus musculus) STRAIN DDY H. Maman Djamaludin* , Iis Inayati Rakhmat** and Sinta Dhea Utami***
* Departemen of Pharmacology
**Departemen of Biochemistry and Molecular Biology
* Faculity of Medical, Jenderal Achmad Yani University
Abstract
Curly red chilies(C annum L) are used for cooking and herbal medicine ingredients.The efficacy has been scientifically proven to have antiinflammatory,anticancer,anticoagulant and antibacterial effects.Needs further testing to find out the safety of its use namely acute toxicity test.The purpose of the study was to assess the degree of toxicity of LD50,changes body weight,organ weight,organ index and macroscopic appearance.This research is an experimental study with Posttest Only Control Group Design method.Total of 24 female and male DDY mice were divided into 4 groups,namely 1control group and 3 test groups.The control group was given feed,drinking water,and CMC while the treatment group was added with EEBCKM at dose of 500 mg/kgbb,1000 mg/kgbb,and 2000 mg/kgbb for 14 days.At 24 hours,counting the number of deaths followed by weighing for 14 days and on the 15th day necropsy was performed to see the macroscopic appearance of the organs weighing organs and organ indexes were obtained from the ratio of body weight to organ weight.The result is we found that there were no deaths in all groups so that the LD50 was above 2000 mgg/kgbb.Statistical analysis showed significant changes in bodyweight with p=0.002and0.003,significant changes in liver and kidney organ weights,significant changes in the index of the liver,kidneys of female and male mice with a value p<0.05,and changes in liver and kidney color at dose of 2000 mg/kgbb and organ enlargement.Administration of it to mice was categorized as relatively non-toxic and showed a delayed toxic effect on the weight loss of female mice and organ index.
Keywords: Capsicum annuum longum, mice DYY strain, toxicity test
