Potential Anti-Acne Ethanol Extract Tuberose Flower (Polianthes tuberosa L.) Against Propionibacterium acnes In Vitro Sayu Putu Yuni Paryati, Awan Buana, Aria Rachmatullah
Faculty of Medicine, Jenderal Achmad Yani University
Abstract
Acne vulgaris is an inflammatory disease of the skin caused by the Propionibacterium acnes. The prevalence of acne vulgaris reached 90% in adolescents to adults in Indonesia in 2016. Tuberose flower (Polianthes tuberosa linn) is a plant with antibacterial properties. Compounds that have antibacterial properties are flavonoids, polyphenols, and steroids. This study aims to determine the anti-acne effectiveness of tuberose flower ethanol extract against the in vitro growth of P. acnes. The design of this study was an experimental laboratory using well diffusion method. Objects of this research were P. acnes ATCC 11827 and the ethanol extract of tuberose flower with a concentration of 5%, 10%, 15%, 20%, 30%, 40%, and 50% with positive control of clindamycin 10 microgram/disk and distilled water as a negative control. Determination of sample repetitions based on Federer^s formula three times. The result at a concentration of 20% was 6.41 +/- 0.11 mm and at a concentration of 50% was 11.61 +/- 0.20 mm. Based on the statistical results of One Way Anova, the value of p = 0.000 and the Post Hoc Tukey test showed that the ethanol extract of tuberose flower concentrations of 5%, 10%, 15%, 20%, 30, 40%, and 50% can inhibit the growth of P. acnes significantly compared to control (p = 0.000). The conclusion is the ethanol extract of tuberose flower (P. tuberosa linn) with concentration of 20% is Minimum Inhibitory Concentration and 50% is an effective concentration in inhibiting the growth of P. acnes bacteria in vitro.
Keywords: Antibacterial, inhibition, polianthes tuberose linn, propionibacterium acnes, tuberose flower
