Acute Toxicity Test of Johar Leaf Ethanol Extract (Cassia siamea.Lamk) in Sprague Dawley Rats Heruna Tanty(a), Tati Herlina(b), Innes Maulidya(c), Rokhana Dwi Bekti(d)
a)Department of Mathematics and Statistic, School of Computer Sciences,
Bina Nusantara University Jakarta 11540, Indonesia
b) Department of Chemistry Faculty of Mathematics and Natural Sciences, Padjadjaran University-Bandung, Indonesia
c) Tropical Biopharmaca Study Center, Bogor Agricultural University, Indonesia.
d)Academic of Industry (AKPRIND),Jogjakarta,Indonesia
Abstract
Cassia siamea.Lamk (Johar) is a tropical plant that grows in various regions in Indonesia. Its bark, leaves, roots, and flowers are traditionally used for the treatment of various ailments, such as itches, malaria, constipation, diabetes, asthma, liver, abdominal pain, heart, etc. Although herbal medicine is generally considered safer to use than synthetic chemical drugs, it is necessary to determine the toxicity for long-term treatment. Therefore, this study aims to determine the acute toxicity of the ethanol extract of C. siamea leaves in Sprague Dawley rats using a fixed-dose method for 14 days. The results showed that the ethanol extract of C. siamea leaves at doses of 300, 2000, and 5000 mg/kgbw did not cause death, symptoms of toxicity, behavioral disorders, changes in body weight, liver and kidneys in rats. Therefore, the LD50 value of C.siamea leaf extract is categorized as non-toxic at a value above 5000 mg/kgbw.
