VIABILITY AND ABNORMALITY OF SPERMATOZOA SEXING ALBUMIN GRADIENT WITH DILUENTS AND ANTIOXIDANT TREATMENT Dian Ratnawati and Fredyansyah Firdaus
beef cattle research station
Abstract
The purpose of this study was to measure the viability and abnormality of sexed spermatozoa from sexing with albumin with the treatment of diluents and antioxidants. The material used in this study was fresh semen of 5 PO bulls with progressive motility more than 70%. The sexing methodology used is 5%, 10% and 15% albumin (egg white) gradients. The diluent treatment used was CEP 2 and andromed with the addition or without the addition of the 1mM antioxidant (glutation). The sexed semen was made in liquid semen and stored at 3 to 5 derajat C. Sample preparation was done by making a smear of spermatozoa on glass objects and coloured with eosin negrosin. Viability and abnormality analysis were performed on days 0 (H0) and 5 (H5). Viability and abnormality analysis of spermatozoa using SCA v.2.1. Parameters measured included spermatozoa viability and abnormality. The design of the experiment used a 2 times 2 factorial pattern. Data were analyzed with SPSS 24. Viability of spermatozoa from sexing using egg white albumin in andromed diluents is better than CEP 2 on storage days 0 to 2. Abnormalities of spermatozoa of sexing during cold storage were not influenced by diluents (CEP 2 and andromed) or the use of glutathione. Viability of spermatozoa during cold storage more than 80% and abnormalities of spermatozoa less than 20% indicate the quality of liquid semen results from sexing is still good.