Screening and Identification Cellulolytic Fungi at Rhizosfer of Safira Taro Plant Eka Wisdawati (a*,b), Tutik Kuswinanti (c), Ade Rosmana (c), Andi Nasruddin (c)
a. The Agricultural Science, Graduate School, Hasanuddin University, Makassar 90245, Indonesia
b. Plantation Department, Pangkep State Polytechnic of Agriculture, Indonesia
c. Departement of Plant Pest and Disease,Faculty of Agriculture, Hasanuddin University, Makassar 90245, Indonesia
*ekawisdawati[at]gmail.com
Abstract
Agriculture waste and pathogen cell wall of plant contain a lot of cellulose which are very difficult to degrade. One of the ways to hydrolyze them is through utilizing cellulase, an enzyme produced from secondary metabolite of cellulolytic fungi. This research was aimed at isolating fungi from rhizosphere of safira taro plant and testing the isolate capability to produce cellulase enzyme to be able to act as decomposer and biocontrol of the plant. The research methods are isolating rhizosphere fungi, testing isolate capability in producing cellulase enzyme and identifying potential isolate producing cellulolytic fungi. It obtained 10 fungi isolates, 4 were negative and 6 positive in producing cellulase enzyme with cellulolytic index 1.16 - 1.66. The highest index was RTB14 isolate which belongs to Aspergillus flavus.
