Thank you for the question.
In this study, the leaves I use are still wet leaves, where I boil the leaves
immediately after they are plucked from the tree without drying. The leaves
that I use are old kersen leaves with the amount of 10 kersen leaves.
From the references I found that both dry and wet kersen leaves still contain
flavonoids
Thank you for the question.
In this study, the leaves I use are still wet leaves, where I boil the leaves
immediately after they are plucked from the tree without drying. The leaves
that I use are old kersen leaves with the amount of 10 kersen leaves.
From the references I found that both dry and wet kersen leaves still contain
flavonoids
bagaimanakah mekanisme daun kersen menurunkan kolsterol?
kandungan zat aktif pada pada kersen yang menyebabkan koresterol turun?
terima kasih atas jawabannya.
Replies:
Mekanisme Kerja Kandungan Daun Kersen
Masing-masing zat aktif dalam daun kersen memiliki mekanisme berbeda sebagai
antibakteri. Flavonoid mampu menghambat fungsi membran sitoplasma,
menghambat sintesis asamnukleat, danmenghambat metabolismeenergi. Saponin
dapat berikatan dengan lipopolisakarida, sehinggamengakibatkan permeabilitas
dinding sel meningkat. Permeabilitas yang terganggu menyebabkan keluarnya
berbagai komponen penting dari sel mikroba yaitu protein, asam nukleat,
nukleotida, dan lain-lain, sehingga sel bakteri akanmati (Sulaiman et al., 2017).
Saponin memiliki aktivitas antioksidan tinggi yaitu dapat menangkal radikal bebas
pada uji DPPH sebesar 80,2% pada 200 ppm dan mampu mengikat kolesterol sebesar
85,07% dibandingkan dengan gemfibrozil yang mengikat 74,83%(Ayu, Mahayani,
Bogoriani, & Bawa, 2019).
Flavonoid mampu menghambat aktivitas enzim HMG-CoA reduktase , sehingga terjadi
penurunan sintesis kolesterol dan kadar kolesterol dalam darah akan menurun(
Prahastuti et al 2011 dalam Winarsih dkk, 2017). Selain itu flavonoid juga dapat
menghambat absorbsi kolesterol di dalam usus, sehingga terjadi penurunan kadar
kolesterol. Absobrsi ini terjadi karena adanya kompleks flavonoid- kolesterol yang
bersifat tidak larut, kemudian berikatan dengan empedu dan membentuk misel
kemudian meningkatkan ekskresi feses(Olivera et al., 2007 dalam Winarsi,
Amurwanto, & Susilowati, 2017).
Daun kersen mengandung senyawa flavonoid, saponin, dan tanin yang
mempunyai daya antibakteri dan antiinflamasi (Sulaiman, Astuti, Dewi, & Shita,
2017).
Senyawa yang terkandung dalam daun kersen (M. calabura L.) dan diduga berperan
dalam penurunan kadar kolesterol adalah flavonoid dan saponin. Berdasarkan
penelitian sebelumnya, saponin dari ekstrak daun kersen dapat membantu
menurunkan kadar kolesterol serta mengurangi penimbunan lemak dalam pembuluh
darah dengan menurunkan tingkat absorpsi kolesterol dan meningkatkan ekskresi
(Lajuck, 2012 dalam Putri dkk, 2018).
Hello Mrs Enik.
I am Reni, in this moment I want ask you about your riset, Your riset is very
good and I think this is Usefull for klien with a high clotesterol, my answer is
how about the control grup that is any intervention? how effectifness kersen
leaves kompare with other material like ginger, garlic or other
Replies:
thanks for the question. the control group was not given any intervention. For kersen
leaves and ginger and garlic, there are can lower cholesterol levels, but it is not yet known
which one is the effectiveness between kersen leaves and them because there is no
research to compare them.
Hello...My Name is Erna Utami
I want to ask you about kersen leaf with garlic, I know that garlic
Can you mix and match for the kersen leaf concoction?
I know that garlic can lower cholesterol, can it be done if only kersen leaves?
sorry, yesterday I didn^t have the chance to see and not be observant if you
have your questions so I only answered today
Respondents who drank kersen leaf stew showed a very significant decrease, so
if I mix kersen leaves with garlic, the thing that I^m afraid of is a very drastic
decrease in cholesterol levels where the cholesterol level is below normal, which
is not good for health.
My question is almost the same as the previous question, have preclinic and
toxicity tests been carried out with certain doses in test animals?
Replies:
Cherry leaf extract has been proven to have significant antioxidant and anti-inflammation
activity, but its ability to
decrease cholesterol level and to prevent vascular inflammation has not been proven. This
study aimed to prove the effect
of cherry leaf extract (EDK) on blood cholesterol level, soluble ICAM-1 level, and aorta foam
cells formation of Wistar rats
fed with high fat and cholesterol diet (HF-HCD). This research used Post Test-Only Effects of
Cherry (Muntingia calabura L) Leaf Extract on Blood Cholesterol, Soluble ICAM-1, and
Foam Cells Formation in High Fat and Cholesterol Fed Wistar Rats
Alvia Nur L , Kis Djamiatun , Martha I Kartasurya
1Magister Ilmu Gizi Fakultas Kedokteran Universitas Diponegoro
Controlled Group Design. As many as 24
male Wistar rats were randomized into 4 groups, including negative control (KN), positive
control (KP), EDK dose 1 (D1), EDK
dose 2 (D2). KN was given a normal diet, KP was given HF-HCD only, D1 was given HF-HCD
and EDK 250mg/kg body weight,
D2 was given HF-HCD and EDK 500mg/kg body weight. Feed and EDK were given once per
day. EDK was given using sonde
together with the feeding for 8 weeks. The results showed that there was a significant
difference (p<0,05) between KN and
KP in HDL cholesterol level. There was a significant difference (p<0,05) between KN and D1
in total cholesterol level. There
was a significant difference (p<0,05) between the KP and D1 at soluble ICAM-1 level. There
were significant differences
(p<0,05) between KP and D2 on total cholesterol, HDL cholesterol, and level of soluble
ICAM-1.
Penelitian intervensi pengobatan dengan metode quasi, belum dapat membatasi
secara ketat faktor lain yang ikut mempengaruhi.
Pertanyaan: Mengapa tidak dilakukan penelitian experimen pada hewan coba agar
dapat dikendlaikan faktor lain yang mempengaruhi
Replies:
sebelumnya sudah ada penelitian yang melakukan eksperimen pada hewan uji yakni
pada 4 kelinci yang diberikan rebusan daun kersen dengan konsentrasi berbeda
didapatkan ada pengaruh pemberian rebusan daun kersen terhadap penurunan glukosa
dengan jumlah yang sesuai dengan konsentrasi yang diberikan.
Untuk mengontrol faktor lain yang dapat mempengaruhi, peneliti membuat buku harian
untuk responden, dimana buku harian tersebut mencantumkan jam tidur, aktivitas dan
pola makan sehingga peneliti dapat mengetahui faktor yang dapat mempengaruhi kadar
kolesterol pada responden.
Based on your presentation, it seems that you have not
explained how many doses of kersen eaf extract are used and
is there a reference dosage used? and I want to know how
does methods to get kersen extract?
Replies:
I am sorry that i am wrong sending the question. My first question should be for
another presenter. I want to ask you about nanoemulsion
because we have the same research background. And my
question are:
1. Is the surfactant (Tween 80) used more than 20% still within
the toxicity limit?
2. surfactants in high concentrations (40%) allow the formation
of micelle. What is your opinion?
i am sorry mrs. Tiara, in here, for this study we did not make an extract but only
boiled 10 pieces of green cherry leaves with 200 cc of water, taken once a day every
day for 7 days
but about kersen ekstract .
The type of research used in this study is a quasi-experimental study using a two-
group pretest-posttest design with a control group design, which consists of an
intervention group and a control group where the intervention group will be given an
action while the control group is only a comparison.
In this study, there were two groups, namely the intervention group and the control
group. At the time of the two groups pretest cholesterol levels will be measured. Then
the intervention group will be given cherry leaf decoction while the control group will
not. After that, the cholesterol levels were measured again in the intervention and
control groups (posttest). Furthermore, the results of the measurement of cholesterol
levels in the pretest and posttest in the two groups were compared. The comparison
results in the intervention group and the control group were compared so that it could
be seen the effect of cherry leaf decoction.
