|
Gold Nanoparticle Biosynthesis using Spirulina plantesis Department of Food Science and Biotechnology, Faculty of Agricultural Technology, Universitas Brawijaya, Jalan Veteran, Malang, Indonesia Abstract Gold nanoparticles (AuNPs) are nanometer-sized gold particles which have diverse applications, including catalysis, sensing, optic, biomedic, anti-cancer agents, anti-microbe agents, and biosensors. Conventionally, synthesizing AuNP typically involves toxic chemicals, intensive energy consumption, and inefficient resource utilization. The formation of AuNPs requires reducing agents to reduce gold ions (Au3+) into metallic golds (Au). Commonly used reducing agents are often environmentally hazardous, thus initiating a greener synthesis method which can be done by using biosynthesis. One promising greener alternative is the use of Spirulina plantesis, which contains proteins, lipids, carbohydrates, fatty acids, polyphenols, and peptides with functional groups. In this research, S.plantesis was cultivated in mixed saline water enriched with KW21 fertilizer, followed by extraction using Ultrasound-Asisted Extraction (UAE). The obtained extract was then used as a substitute for reducing agent in the turkevich method. The research primarily focused on the effect of pH conditions, volume ratio of S.plantesis extract to HAuCl4, and S.plantesis extract concentration on AuNPs particle formation. Preliminary results showed that AuNPs synthesized using S.plantesis exhibited absorption peaks at 535 nm, thus confirming the AuNPs formation. Further research will be done to characterize the AuNPs formation using Scanning Electron Microscope, Particle Size Analyzer, and Fourier Transform Infrared Spectroscopy. The synthesized AuNPs are expected to be spherical, monodisperse, and within a size below 100 nm. Keywords: AuNPs- Biosynthesis- Gold Nanoparticle- Green Technology- KW21- Spirulina plantesis Topic: Food science and biotechnology |
| ICGAB 2025 Conference | Conference Management System |