Bioactive Metabolite Profiling of Angelica keiskei Leaves by LC-MS for the Discovery of Anti-Aging Marker Compounds
Arie Febrianto Mulyadi*, Adinda Indrawati Subagyo, Jaya Mahar Maligan

Agricultural Technology Faculty, Universitas Brawijaya
Jl. Veteran, Malang, Indonesia
*Email: arie_febrianto[at]ub.ac.id

Abstract

Angelica keiskei (Ashitaba) is a medicinal plant traditionally valued for its health-promoting properties, including anti-aging potential. This study aimed to profile bioactive metabolites from ashitaba leaves using liquid chromatography-mass spectrometry (LC-MS) and to identify marker compounds associated with anti-aging activity. Ethanolic leaf extract was analyzed with LC-MS, detecting 133 compounds comprising flavonoids, chalcones, phenolics and terpenoids. Relative quantification based on peak area indicated that kaempferol, luteolin, chrysin, quercitrin, xanthoangelol, and 4-hydroxyderricin were dominant compounds with documented antioxidant, anti-inflammatory, and cellular senescence-modulating effects. Literature review linked these metabolites to key anti-aging pathways such as Nrf2, SIRT1, and AMPK activation. The findings highlight ashitaba leaves as a rich source of multifunctional bioactives and propose specific compounds as chemical markers for future pharmacological validation. This LC-MS-based profiling provides a foundation for developing ashitaba-derived anti-aging formulations.

Keywords: Angelica keiskei- Anti-aging- Bioactive compound- LCMS- Marker compound

Topic: Food science and biotechnology