Biodiversity of Cellulolytic Bacteria from Termite Gut Isolated from Bengkulu Coastal Area Dwita Oktiarni (1,2*) and Lusiana(1)
(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Bengkulu, Jalan W.R.Supratman, Kandang Limun, Kota Bengkulu, 38122, Indonesia.
*dwita.oktiarni[at]unib.ac.id
(2) Research Center of Sumatera Natural Products and Functional Materials, Universitas Bengkulu, Jalan W.R.Supratman, Kandang Limun, Kota Bengkulu, 38122, Indonesia.
Abstract
The termite from Bengkulu coastal area was classified as a high-level termite. Termites, being highly social insects, break down cellulosic biomass into glucose using bacteria that produce cellulolytic enzymes in their guts. Identification of the new species of cellulolytic bacteria from temite was performed by biomolecular assay. The bacteria isolates were isolated and purified by using DNA kit and the optical density of bacterial DNA isolates was carried out by using nanodrop spectrophotometer. The isolated bacterial strains were amplified by PCR to determine the 16S rRNA gene segment, performed using BLAST-N program and compared with NCBI database.
