Ethanolic Extract of Guava Seed Leaves (Psidium guajava L.) as Media for Biosynthesis of ZnO/CuO Nanoparticles : Characterization and In Vitro Study to Staphylococcus aureus Bacteria Ahmad Fatoni a,*, Khoirunnisa Sari b, Lasmaryna Sirumapea a and Nurlisa Hidayati c
aPharmaceutical Chemistry, Bhakti Pertiwi High School of Pharmacy Science, Palembang,
South Sumatera Indonesia 30128
bUndergraduate of Bhakti Pertiwi High School of Pharmacy Science, Palembang, South Sumatera Indonesia 30128
cDepartment of Chemistry, Faculty of Mathematic and Natural Sciences, Sriwijaya University,
Indralaya Ogan Ilir South Sumatera, Indonesia
Abstract
Research was done on ZnO/CuO nanoparticle biosynthesis. The objective of this study was to synthesize ZnO/CuO nanoparticles, characterize them, and use them as an antibacterial agent against Staphylococcus aureus. The media of ethanolic extract of guava seed leaves (Psidium guajava L.) in alkaline solution were used to biosynthesize ZnO/CuO nanoparticles. The tools used to examine the functional groups and physical structure of ZnO/CuO nanoparticles include FTIR spectroscopy and X-ray diffraction. Using the well agar diffusion method, ZnO/CuO nanoparticle suspension was used as an antibacterial agent. Wavenumbers for the Zn-O and Cu-O groups at ZnO/CuO nanoparticles are 507.28 and 617.22 cm-1, respectively. ZnO/CuO nanoparticles^ crystallite size was calculated to be 2.33 nm. Staphylococcus aureus may be resistant to the ZnO/CuO nanoparticles^ antimicrobial effects. ZnO/CuO nanoparticles have a greater inhibitory zone than ZnO or CuO nanoparticles.
