TESTING THE INHIBITION ACTIVITY OF THE ALPHA-GLUCOSIDASE ENZYME OF MELINJO LEAF (Gnetum gnemon L.) EXTRACT AND FRACTIONS IN VITRO
Herlina Herlina1*, Elfita2

1 Departement of Pharmacy, Faculty of Mathematics and Natural Sciences, Sriwijaya of University
2 Departement of Chemistry, Faculty of Mathematics and Natural Sciences, Sriwijaya of University

Abstract

Diabetes mellitus (DM) is a serious chronic metabolic disorder characterized by high blood sugar levels (hyperglycemia) and can trigger various complications. Therapy for oral antidiabetic uses drugs that inhibit the enzyme &#945--glucosidase. This study aims to examine the antidiabetic effectiveness of melinjo leaf (Gnetum gnemon L.) extracts and fractions through in vitro inhibition of the &#945--glucosidase enzyme. The inhibition test was carried out by measuring the absorbance of p-nitrophenol produced as a product of the enzymatic reaction at a wavelength of 405 nm using a microplate reader. The IC50 value for ethanol extract, ethanol fraction, ethyl acetate fraction, and n-hexane fraction was 589.39- 494.51- 99.61, and 56.82. The ethyl acetate and n-hexane fractions have a better potential to inhibit the &#945--glucosidase enzyme than the ethanol extract and ethanol fraction. From phytochemical tests, it was found that the ethanol extract and ethanol fraction contained alkaloids, flavonoids, tannins, steroids, and saponins, the ethyl acetate fraction contained flavonoids and tannins while the n-hexane fraction contained steroids. The ethyl acetate fraction and n-hexane fraction have the potential to inhibit the A-glucosidase enzyme

Keywords: melinjo leaf (Gnetum gnemon L.), &#945--glucosidase enzyme, IC50, n-hexane fractionPlease Just Try to Submit This Sample Abstract

Topic: Pharmacy

Plain Format | Corresponding Author (Herlina Herlina)

Share Link Copy Text

Share your abstract link to your social media or profile page