Antibacterial Activity of Semprawang (Dillenia ochreata) Leaves and Stem Bark Against Escherichia coli and Staphylococcus aureus
Restri Diah Carissa

Chemistry Faculty Mathematics and Natural Science, Sriwijaya University

Abstract

Semprawang (Dillenia ochreata) is one of the traditional medicinal plants of the
Dilleniaceae family for wound treatment. It is known that the chemical content and biological
activity of the D. ochreata plant is still very limited. The aims of this study were determine the
antibacterial activity of the total methanol extract and the fractions (n-hexane, ethyl acetate and
methanol) from the leaves and stem bark of D. ochreata. The antibacterial activity on E. coli and
S. aureus bacteria was carried out by diffusion method and followed by determination of minimum
inhibitory concentration (MIC) by liquid dilution method. Extraction process was conducted using
maceration method with methanol as solvent and to obtained fraction using maceration with step
grader priority (n-hexane, ethyl acetate and methanol). This research showed that the total
methanol extract of semprawang leaves was more active than the steam bark methanol extract
against both E. coli and S. aureus bacteria with the diameter of inhibition zone at 8.87 mm and
7.35 mm respectively. The MIC value for each total extract and stem bark and leaf fraction of D.
ochreata up to a test concentration of 125 &#956-g/mL showed that only the n-hexane fraction against
E. coli and S. aureus had the highest difference in MIC values of 75 &#956-g/mL and 125 &#956-g/mL
respectively. The MBC test, only D. ochreata stem bark of n-hexane fraction against E.coli
bacteria were able to provide bactericidal with a MBC value of 125 &#956-g/mL.

Keywords: Antibacterial, Dillenia ochreata, Leave, Stem bark, E. coli, S.aureus

Topic: Chemistry and Applied Chemistry