OPTIMIZATION OF CELLULASE PRODUCTION BY Chaetomium globosum 17BDSM USING SOLID PHASE FERMENTATION METHOD WITH BAGASSE, RICE STRAW AND CORN COBS
Elisa Nurnawati, Rasti Puspha Amrina Rosyada, Muharni, Hary Widjajanti

Department of Biology, Faculty of Mathematics and Natural Science, Sriwijaya University

Abstract

Cellulose is still present in agricultural by-products, although it hasn^t been fully utilised yet. Fungi and bacteria are examples of microorganisms that can manufacture cellulase enzymes. The fungus Chaetomium globosum 17BDSM possesses cellulolytic properties. The following variables affect cellulase activity: pH, temperature, thickness, and substrate. The goal of this research is to determine the ideal substrate thickness, pH, and temperature in order to maximise C. globosum 17BDSM^s growth function and produce high-activity cellulase. This study treated substrate type, temperature, pH, and substrate thickness using a factorial Randomised Block Design (RAK) experimental design. Based on the research results, cellulase activity is impacted by the thickness treatment- at 1.5 cm of thickness, cellulase activity is 0.153981U/mL. Cellulase activity was influenced by the kind of substrate treatment, specifically sugarcane bagasse and corncob substrates, which had respective cellulase activity values of 0.321773 U/mL and 0.039816 U/mL. Treatments with pH and temperature had no effect on cellulase activity.

Keywords: Cellulase, Chaetomium globosum, optimization

Topic: Biology and Applied Biology