SUNGKAI (Peronema canescens JACK) LEAF METHANOL EXTRACT FOR PROLIFERATION OF Saccharomyces cerevisiae YEAST AND MICE (Mus muculus) MICE
Irena Francina Kelly Kamal (a*)- Hermansyah Hermansyah (a)- Desnelli Desnelli (a)- Muharni Muharni (a)- Yosie Andriani (b)

a) Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Sriwijaya, Jalan Raya Palembang Prabumulih, Ogan Ilir, Sumatera Selatan
*08031381924100[at]student.unsri.ac.id or kellylily101201[at]gmail.com
b) Institute of Marine Biotechnology, Universiti Malaysia Terengganu

Abstract

Sungkai (Peronema canescens Jack.) is one of woody plants which is usually found in the Southern part of Sumatra which is often used as an herbal plant by people for flu, toothache, and bathwater for postpartum mothers. This research was aimed to know immunomodulator potency of Sungkai in mice (Mus muculus) and effect extract to proliferation of Saccharomyces cerevisiae yeast and leukocytes. Yeast proliferation is associated with cancer cells while leukocytes assay is associated closely with immunostimulatory activity. Sungkai leaf was macerated using methanol then evaporated and tested for its phytochemistry profile and thin layer chromatography. Several groups were detected in phytochemistry profiling, such as terpene, steroid, alkaloid, saponin, and flavone. For the leukocytes assay of the extract, mice (Mus musculus) were used. There were several variables, positive control (Stimuno), negative control (CMC-Na), 5% concentration, 10% concentration, and 15% concentration. Mice leukocytes were taken from tail biopsy and counted under haemocytometer and microscope. 15% concentration extract results in the highest content of leukocytes found in the mice tail which was 9560 units, while negative control results in the lowest content of leukocytes which was 5250 units. This indicated that sungkai extract has potency as immunomodulator. This extract was then tested for its proliferation activity of S. cerevisiae yeast growth by adding some extract of various concentrations, 5%, 10%, 15%, and 20%, to yeast culture broth. Based on optical density observation using spectrophotometer UV-Vis (&#955- = 660 nm) show that there was no significant difference cell growth among in the absence and in the presence of various concentrations of extract. This indicated that sungkai has no effect for the proliferation of S.cerevisiae cell.

Keywords: sungkai, proliferation, leukocytes, immunomodulator

Topic: Chemistry and Applied Chemistry