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Antioxidant and Antibacterial Activities of Ethyl Acetate Extract of Endophytic Fungi Isolated from Ciplukan (Physalis angulata L.) Fruit 1 Postgraduate School of Sciences, Faculty of Mathematics and Natural Sciences, Sriwijaya University. Jl. Padang Selasa No. 524, Palembang 30129, South Sumatra, Indonesia Abstract Physalis angulata L. well known traditionally in treating various diseases caused by free radicals and bacterial infections. Endophytic fungi in P. angulata L. plants are of concern because they have the potential to be developed as an alternative for providing medicines. This study aims to identify morphological characters and investigate the antioxidant and antibacterial activity of ethyl acetate extract of endophytic fungi from P. angulata L fruit and compare it with the host plant. Identification of fungal morphology based on macroscopic and microscopic characters. Antioxidant activity was tested using the DPPH method and antibacterial activity was tested using the Kirby-Bauer diffusion method. The results of the isolation of endophytic fungi from P. angulata L. fruit resulted in 2 isolates, namely CH1 and CH2. Based on macroscopic and microscopic characters, isolate CH1 was identified as Fusarium sp. and isolate CH2 was identified as Pythium sp. The endophytic fungal isolate was then cultivated to obtain ethyl acetate extract. The antioxidant activity of the isolated endophytic fungus ethyl acetate extract was weaker than the host plant, while the antibacterial activity was stronger than the host plant. This research shows that endophytic fungi isolated from P. angulata L. fruit have the potential to be developed into medicinal ingredients to treat free radicals and bacterial infections. Keywords: Endophytic fungi, Physalis angulata L., antioxidant, antibacterial Topic: Biology and Applied Biology |
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