Thin Layer Chromatography Analysis and Antibacterial Activity of Ecoenzyme Solution
Ratna Dewi Eskundari (a*), Agus Purwanto (a), Nur Rokhimah Hanik (a), Tri Wiharti (a), Suwaji Handaru Wardoyo (b)

Biology Education Study Program, Universitas Veteran Bangun Nusantara, Sukoharjo 57521, Indonesia
Acupuncture and Herbal Medicine Study Program, Politeknik Kesehatan Kementerian Kesehatan Surakarta, Surakarta 57127, Indonesia
* ratnadewi[at]univetbantara.ac.id

Abstract

Ecoenzyme is known as fermented organic garbage solution that can be used for everyday purposes such as organic liquid fertilizer and antiseptic. Until now, no information has been reported regarding further phytochemical tests and antibacterial activity of ecoenzyme solution. Based on these facts, it was deemed necessary to carry out this research in order to obtain scientific information regarding phytochemical activity using thin layer chromatography (TLC) as well as antibacterial activity contained in ecoenzyme solution. Initial screening for phytochemical content was carried out by testing alkaloids compound using the Wagner method, testing flavonoids and saponins compound using strong acid method. Further phytochemical tests were carried out using TLC using a silica gel plate with mobile phase, i.e. Methanol: ammonia for alkaloid test, n-hexane: ethyl acetate: formic acid for flavonoid test, and chloroform: methanol for saponin test. Antibacterial activity test was carried out using disc method. Initial screening results on the phytochemical content of ecoenzyme solutions showed that three ecoenzyme solutions tested did not contain saponins. The TLC results showed that ecoenzyme solution derived from skin of 3 types of fruit contained flavonoids and saponins, also this ecoenzyme has the best antibacterial activity against gram-positive and negative bacteria.

Keywords: antibacterial- ecoenzyme- TLC

Topic: Life Sciences