THERAPEUTIC ACUPUNCTURE AND METHYLPREDNISOLONE EFFECTS TO CORTISOL LEVEL IN MICE WITH LUPUS NEPHRITIS MODEL
Suparyanti, E.,L., Purwanto, B., Wasita, B., and Handayani, S.

Sebelas Maret University

Abstract

Systemic Lupus Erythematosus (SLE) is an autoimmune disorder characterized by multiorgan involvement and various autoantibody with the target of cell components. One of the clinical manifestations of SLE is lupus nephritis. Acupuncture is one of the therapeutic methods that has a philosophy of self-healing potential, the ability of the body to heal itself. This study aims to understand the therapeutic effects of acupuncture points of Mingmen (GV4) and Zusanli (ST36) and methylprednisolone in mice with lupus nephritis model comparing cortisol level. This study is an experimental study on mice (Mus musculus) modeled with lupus nephritis using post-test only on control group design. The subjects were assigned into four groups, each with seven mice, consisting of control group (CN), negative control group given pristane (C(-)), first test group given pristane and methylprednisolone (P1), and second test group given pristane and acupuncture in Mingmen and Zusanli points. Significant p < 0,05. Result of this study found that there
was a statistically significant difference between groups as demonstrated by one-way ANOVA test (F=11.710, p-value of 0.000). A Tukey post hoc test showed that the mice given pristane (C(-)) were found to have higher cortisol than P1 group (p-value of 0,000), P2 group (p-value of 0,025), and the control group (CN group) (p-value of 0,000). There was no significant difference found in control group (CN) with P1 group (p-value of 0,888), control group (CN) with P2 group (0,112), and P1 and P2 group (p-value of
0,374).

Keywords: acupuncture, mingmen point, and zusanli point, methylprednisolone, lupus nephritis

Topic: Medical : basic science, clinical, translational research, medical education, and miscellaneous