PROPOLIC EFFECT OF MOUNT LAWU ON EXPRESSION VASCULAR ENDOTHELIAL GROWTH FACTOR IN ACUTE RINOSINUSITIS MODEL MOUSE INDUCED BY ESCHERICHIA COLI
Viquisa Berliana Putri AZIZA1*, Sabdo Nugroho WINULANG1, Hendradewi SARWASTUTI1, Sudrajad HADI1

1 Department of Otorhinolaryngology Head and Neck Surgery, Dr.Moewardi General Hospital Surakarta, Indonesia

Abstract

Background: Rhinosinusitis is an inflammatory disease of the nasal mucosa and paranasal sinuses. Bacterial rhinosinusitis caused by E.coli can increase the Vascular Endothelial Growth Factor (VEGF). Propolis can be considered an alternative therapy for rhinosinusitis. Objective: To determine the propolis effect on the expression of VEGF in acute rhinosinusitis model mouse induced by Escherichia coli. Methods: An Experimental study with post-test only control group design. The sample was 40 male white mouse SDF Sprague-Dawley which were divided into five groups, P1 group (control), P2 group (acute rhinosinusitis mouse model), P3 group (acute rhinosinusitis mouse model + amoxicillin 200 mg/kgBW), group P4 (mouse model acute rhinosinusitis + propolis 100 mg/kgBW) and group P5 (mouse model acute rhinosinusitis + propolis 200 mg BW). They were induced with right and left intranasal E. coli and 0.1 ml intraperitoneal each with a concentration of 3x109 colony forming units (CFU) ml for 28 days. On days 29 to 42, groups P3, P4, and P5 were treated with amoxicillin and propolis. On day 43 the subjects were turned off, followed by VEGF expression measurement. Result: Data analysis using Kruskal-Wallis test showed insignificant results (p=0,248). The VEGF expression at the beginning was high in P1, but in P2, P3 and P4 didn^t decrease VEGF expression, while P5 reduced VEGF expression but it was not significant. Conclusion: There was no effect of Mount Lawu propolis extract on VEGF expression in acute rhinosinusitis model mouse induced by Escherichia coli.

Keywords: Propolic of mount lawu, echerichia coli, VEGF, acute rhinosinusitis

Topic: Medical : basic science, clinical, translational research, medical education, and miscellaneous