Increased level of TNF-&#945- in serum and peritoneal fluid of endometriosis women-related infertility
Nanda Yuli Rahmawati1, Fadhil Ahsan2, Budi Santoso2, Alfin Firasy Mufid1, Ashon Sa^adi2, Sri Ratna Dwiningsih2, Arif Tunjungseto1,2, M. Y. Ardianta Widyanugraha1,2

1Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.
2Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia.

Abstract

Objective: TNF-&#945- has been implicated in developing endometriosis- however, TNF-&#945- is largely unexplored in women with infertility cases. We aimed to evaluate serum and the peritoneal fluid level of TNF-&#945- in endometriosis patients and correlates its level to the severity of endometriosis.
Study design: Peripheral blood and peritoneal fluid samples were obtained from 87 infertility cases who underwent laparoscopy, consisting of 44 endometriosis women and 43 non-endometriosis women. We assessed serum and peritoneal fluid levels of TNF-&#945- using enzyme-linked immunosorbent assay.
Results: Serum and peritoneal fluid TNF-&#945- levels were significantly increased in the endometriosis group compared to the control group (p-value = 0.0278- p-value = 0.0271). Serum and peritoneal TNF-&#945- levels were significantly different between late-endometriosis and the control group (p-value = 0.026, p-value = 0.004).
Conclusions: Our results show increased levels of TNF-&#945- level in endometriosis women related to infertility cases that correlate with the severity of endometriosis.

Keywords: endometriosis, infertility, the severity of endometriosis, TNF-&#945-

Topic: Medical : basic science, clinical, translational research, medical education, and miscellaneous