Effect of Chitosan Nanoparticles from Red Snapper Scales (Lutjanus Sp) on Pulp Repair (In Vivo Study)mit This Sample Abstract
Noor Hafida Widyastuti1, Adi Prayitno2, Risya Cilmiaty3, and Brian Wasita4

Doctoral Program of Medical Science, Program of MedicalScience, Faculty of Medicine, Universitas Sebelas Maret

Abstract

Exposed pulp due to iatrogenic action can cause pulp inflammation. Reversible pulpitis is a mild to moderate inflammatory condition that can be treated with pulp capping treatment. Pulp capping treatment can support pulp repair by forming reparative dentin. The pulp caping material determines the success of the treatment. Calcium hydroxide (Ca(OH)2) is the gold standard material for the treatment but has many drawbacks. Chitosan has antibacterial and anti-inflammatory activity. Red snapper scales contain chitin which can be processed into chitosan nanoparticles. This study aims to determine the effect of chitosan nanoparticles (CN) from Red Snapper Scales (Lutjanus Sp) on pulp repair (identify from the expression of TGF-&#946-, the number of odontoblast like-cells, the formation of reparative dentin). This research laboratory is done in vivo using 24 samples of Sprague Dawley rats. The study uses four groups there are healthy rat groups, reversible pulpitis, reversible pulpitis applied the Ca(OH)2 and reversible pulpitis group applied CN. Observations of expression of TGF-&#946- using ELISA, odontoblast-like cells and dentine reparative formation using the histopathological examination. The results showed that one-way Anova Test for TGF-&#946- expression obtained p=0,049 (p<0.05). Kruskal Wallis test for odontoblast like-cell obtained p=0,006 (p<0,05) and for dentin reparative formation p=0,009 (p<0,05). This means that there is a significant difference between groups. Based on the results of the study, it can be concluded that red snapper scales chitosan nanoparticles enhance TGF-&#946- expression on of odontoblast-like cells and dentine reparative formation in the pulp repair process

Keywords: chitosan nanoparticle, TGF-&#946-, Pulp repair, Dentin reparative

Topic: Medical : basic science, clinical, translational research, medical education, and miscellaneous