THE INFLUENCE OF Cissus quadrangula Salisb EXTRACT TO BONE LENGTH AND THE NUMBER OF OSTEOBLAST IN FEMUR OF DDY MICE
Hendri Priyadi, Fransiska Ambarukmi Pontjosudargo, Irmadella, Alif Pascal

Fakultas Kedokteran Universitas Jenderal Achmad Yani

Abstract

Cissus quadrangularis have been proven to have an influence on bone growth and the healing process in fractures and prevention of osteoporosis. Cissus quadrangularis has been widely used as traditional medicine and has proven to increase osteoblast activity, and could accelerate the recovery of bone fractures. The aim of this study was to assess the effect of the stem of Sipatah-patah (Cissus quadrangularis) on the growth of bone length and the number of osteoblast cells in mice femur. This study used a comparative method with a completely randomized design of 24 mice which were divided into 4 groups. P0 is a control group that is only given standard food, while P1 to P3 are the treatment groups given Cissus quadrangularis extracts at doses of 500 mg/KgBW, 700 mg/KgBW and 900 mg/KgBW. The extract was given for 30 days, then measured the length of the bone and the number of osteoblasts on the metaphyseal plate of mice femur. Data were analyzed using One Way ANOVA and Post Hoc Duncan^s test with a 95% degree of confidence. The results showed that Cissus quadrangularis extract at a dose of 700 mg/KgBW and 900 mg/KgBW had an effect on femur length and the number of osteoblasts was significantly higher (p <0.005) than the control group. It can be concluded that Cissus quadrangularis extract has the effect of increasing the length growth and number of osteoblasts Os. Femur in DDY strain mice.

Keywords: Cissus quadrangular Salibs, bone length, osteoblast, femur

Topic: Biomedical Science