The Effectiveness of Flavonoids From The Ethanol Extract of Daun Dewa (Gynura Divarycata L) to Increase The Number of Pancreatic Beta Cells in The Pancreatic Gland Through Histopathological Observations
Dian Anggraeny, Mutia Susparini Subando, Ris Kristiana, Raynisa Budhy Pratiwi

Jenderal Achmad Yani University


Abstract

Diabetes mellitus (DM) is a metabolic disease characterized by increased blood glucose levels. The condition of hyperglycemia that occurs continuously can increase oxidative stress, resulting damage to the pancreatic beta cells. Glibenclamide treatment often used in the treatment of diabetes mellitus. However, glibenclamide cannot stimulate an increase in the number of pancreatic beta cells if its damage. Nowadays many traditional treatments are searched to treat pancreatic beta cell damage. One of them uses ethanol extract of DaunDewa which has antioxidant and hypoglycemia effects. This study aims to prove that the administration of ethanol extract of DaunDewa can have an effect on decreasing blood glucose levels and increase the number of pancreatic beta cells in people with diabetes mellitus. This research is an experimental study with a post test only control group design. The research subjects used were 24 DDY mice divided into four groups consisting of negative control (K1), positive control (K2) (alloxan 150mg/kgBB), ethanol extract of DaunDewa(K3) (400mg/kgBB), Glibenclamide (K4) (0.64mg/kgBB). Data were analyzed using the Post HocTukeytest to see meaningful differences between groups. The results showed significant differences (p,0.05) between the group ofethanol extractof the DaunDewa with thepositive group (p value=0.000) and significant differences between theethanol extractof DaunDewagroupand the glibenclamide group (p value=0.001). But there was no significant difference (p>0.05) between the group of ethanol extract of the DaunDewa with thenegative group (value p=0.991). Significant differences show that ethanol extract can reduce blood glucose levels by increasing the number of pancreatic beta cells that have been induced by alloxan. The active component of flavonoids in the ethanol extract of DaunDewa acts as an antioxidant has been effective in increasing the number of pancreatic beta cells.

Keywords: Diabetes Melitus, Ethanol extract of DaunDewa, Pancreaticbeta cells

Topic: Biomedical Science

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