The Comparison of Ethanol Extract Breadfruit Leaf and Glibenclamide Towards the Decreasing of Blood Glucose Levels in Rats with Diabetes Mellitus Induced by Aloxan
Iis Inayati Rakhmat, Danial Ahmad Dzulfikri, Hadi Jusuf

Medical Faculty, Universitas Jenderal Achmad Yani

Abstract

Diabetes mellitus is a disease that will increase in the future. There are many drugs used to treat this disease, but they have a bad side effect when they used for long term. Breadfruit^s leaf (Artocarpus altilis) is one of many plants that usually used as an antidiabetic by Indonesian people, because it contains quercetin, a flavonoid that can be used as antidiabetic and antioxidant. This study aimed to determine the effect of ethanol extract of breadfruit^s leaf with glibenclamide on blood glucose levels of male albino rats (Rattus norvegicus) of wistar strain induced by alloxan. This research was conducted with experimental methods using 35 male albino rats of wistar strain. They were divided into 5 groups, 1 group as a negative control, and 4 other groups induced by alloxan 120 mg/kgBW, further divided into positive control, the treatment group ethanol extract of breadfruit^s leaf 100 mg/kgBW, the treatment group glibenclamide 0,45 mg/kgBW, and the combination treatment group ethanol extract of breadfruit^s leaf 100 mg/kgBW with glibenclamide 0,45 mg/kgBW. Treatment was given for 21 days and blood glucose levels checked in the day before treatment and after treatment, to obtain the difference in blood glucose levels. The result statistical analysis of research by One-Way ANOVA test was significantly different after 21 days of treatment (p <0.05). Then continued by Post Hoc Tukey test results showed that there were not significantly different among 3 treatment groups. This happened because the breadfruit^s leaf had quercetin as an antidiabetic and antioxidant, while glibenclamide had the effect of increasing the secretion of insulin, then blood glucose levels would decrease. In conclusion ethanol extract of breadfruit^s leaf, glibenclamide and the combination treatment group capable of lowering blood glucose levels, but there were no significant differences between three treatment groups.

Keywords: antidiabetic, breadfruit^s leaf, diabetes melitus, flavonoid, quercetin

Topic: Biomedical Science