ANTIHYPERURISEMIC EFFECTIVITY STUDY OF ETHANOLIC EXTRACT OF BREADFRUIT LEAVES (Artocarpus altilis) ON MICE (Mus musculus) DDY STRAIN, HIGH PURINE DIET INDUCED
Maman Djamaludin*, Daswara Djajasasmita**, Nabila Khoerunnisa***

*Laboratorium Farmakologi,**Laboratorium Fisiologi,***Progam Studi Kedokteran
Medical Faculty of Universitas Jenderal Achmad Yani

Abstract

Hyperuricemia is a condition with increased in blood uric acid level . This condition causes pathological abnormalities in the form of a build up of monosodium urate crystals in body tissues. Provision of herbal medicines, one of which is of bread fruit leaves (Artocarpus altilis) thought to reduce blood uric acid level. This is the antihyperuricemic effectivity test of breadfruit leaves (Artocarpus altilis) ethanol extract against mice induced by chicken liver juice. Chicken liver juice dose of 50 mg / kgBW induces an increase in uric acid level so that it becomes hyperuricemic. The study was conducted with an experimental method with mice of 24 DDY strain which divided into 6 groups. The treatments consisted of: Negative control (G-I), positive control (G-II), admnistered of breadfruit leaves ethanol extract dose 150 mg / kgBB (G-III), dose 300 mg / kgBW (G-IV), dose 600 mg / kgBW (G-V) and allopurinol dose 13 mg / kgBW (GVI).The whole group was acclimatized for 7 days and given test material on days 8-16, on the 17 th day all groups were drawn from retro orbital veins and checking for uric acid levels. The results of the study using the Post Hoc Tukey Test showed that the level of uric acid in all treatment groups were significantly different (p <0.05). From these results it was seen that ethanol extract of breadfruit leaves (Artocarpus altilis) at a dose of 600 mg /kgBW was the most effective dose to reduce uric acid level compared to 150 mg / kg BW and 300 mg /kgBW, breadfruit leaves ethanol extract 600 mg /kgBW has the same effectivity as allopurinol dose 13 mg /kgBW. This is because breadfruit leaves have secondary metabolites namely flavonoids, alkaloids, and tryptenoids which can inhibit the xanthine oxidase enzyme so that uric acid in the blood decreased.

Keywords: breadfruit leaves, chiken liver juice, hyperuricemia, allopurinol

Topic: Biomedical Science