THE EFFECT OF FAT COMPOSITION TO LIVER ENZYMES, CHOLESTEROL TRIGICERIDE AND LIVER MDA LEVELS IN WISTAR STRAIN RATS WHICH IS INDUCED BY HIGH FAT DIET
Fransiska Ambarukmi1, Hendri Priyadi2, Evi Soviai3, Lia Siti Halima4, Endry Septiadi5

Fakultas Kedokteran Universitas Jenderal Achmad Yani

Abstract

Research to assess liver damage has been widely studied, but intervention models of liver damage still use hepatotoxic substances. The important thing in research on Non Alcoholic Fatty Liver Disease, is that it is difficult to compare the results of the studies, because the variety of studies is very diverse. The description of diet composition that uses fat content as the main source of calories is neglected. The target of this study is to obtain scientific information regarding the composition of fat diet which can be used to induce dyslipidemia and liver damage. The study used 15 rats with the number of replications for each group was 3 rats in 5 groups consisting of negative controls, groups that were given a diet of 50%, 60%, 70% and 80% fat for 2 weeks. The indicators assessed were lipid profiles including triglyceride levels, total cholesterol, SGPT enzyme levels and liver MDA. The data obtained were analyzed using One Way Anova and Duncan^s test, with a confidence degree of 95%. The results showed that giving a high-fat diet with a composition of up to 80% fat did not result in a significant increase in triglyceride levels, but resulted in a significant increase in liver cholesterol, SGPT and MDA levels. The diet with a composition of 50% fat for 2 weeks increased cholesterol and liver MDA levels which was significantly different from the control group, whereas a significant increase in SGPT levels was seen in the group given a 70% high-fat diet. Giving a high-fat diet for 2 weeks can increase cholesterol, SGPT enzymes and liver MDA levels, but is not able to increase triglyceride levels significantly.

Keywords: High fat diet- Cholesterol- Liver MDA- SGPT- Triglyceride

Topic: Biomedical Science