EFFECT OF ETHANOL EXTRACT OF ROBUSTA LAMPUNG COFFEE ON THE FUNCTION OF THE LIVER AND KIDNEY OF WISTAR RAT
Dewi Ratih Handayani1, Aditya Gilang Nur Pratama2, I Wayan Agus Putra2

1Department of Biochemestry, Faculty of Medicine,Universitas Jenderal Achmad Yani, Faculty of Medicine, Universitas Jenderal Achmad Yani

Abstract

Phytochemical tests of coffee show that coffee contains antioxidant compounds such as caffeine and chlorogenic acid, which play a role in the healing process of disease. However, it is necessary to know the safe dosage of coffee extract use in treating diseases. The purpose of this study was to determine the effect of ethanol extract of Robusta Lampung coffee beans on plasma of AST,ALT, urea, and creatinine of Wistar rats.
This study used white male Wistar rats which were divided into Group K (control), Group P1 was given ethanol extract of Robusta coffee beans (EECB) with a dose of 625 mg/Kg BW, Group P2 with a dose of 1250 mg/Kg BW. Group P3 dose 2500 mg/Kg BW, Group P4 dose 5000 mg/Kg BW. Observations were made from the 1st to the 14th day, on the 15th day ALT, AST, urea, and creatinine were examined.
The results showed an increase in the mean levels of ALT, AST, and urea in the P1-P4 group, and there was a significant difference using the ANOVA test with p-value <0.05. Creatinine levels did not show any difference. Tukey^s PostHoc Test, AST levels were significantly increased in the P3 and P4 groups, while ALT and urea increased in the P4 group. The conclusion of this study is that ethanol extract of Robusta Lampung coffee beans, a dose of 2500 mg/Kg BW increases plasma AST levels, while a dose of 5000 mg/Kg BW increases levels of ALT, AST, and urea.

Keywords: ALT, AST, coffee, creatinine, urea

Topic: Biomedical Science