The growth curve and total colonies number of Lactobacillus sp on the red guava (Psidium guajava Linnaeus) juice medium
Eka Noneng Nawangsih, Putri Heryani Kusnawan, Maman Djamaludin

Faculty of Medicine, Universitas Jenderal Achmad yani

Abstract

Probiotics are bacteria that are beneficial to human health. Probiotic drinks from cow milk medium use carbohydrates, vitamins and minerals for its fermentation process. Red guava fruit has a higher content of fructose, glucose, sucrose, vitamins (B1-B3) and phosphorus and is non-cholesterol than cow milk. This study aims to find out the total number of colonies and an overview of the growth curve of Lactobacillus sp cultured in the medium of red guava juice (Psidium guajava Linnaeus) in vitro and then compared with the Indonesian national standard for probiotic drinks (107 CFU/mL). The growth of Lactobacillus sp in red guava medium observations every 12 hours for 60 hours. The method used for calculating the number of colonies is the TPC (Total Plate Count) method then described in the form of a growth curve. The results showed the growth curve Lactobacillus sp in the media of red guava juice from the log phase until the death phase starting at the 12th hour, 24, 36, 48 to 60 with TPC amounts of 21.5x1010CFU/mL, 49x1010CFU/mL, 49.5x1010CFU/mL, 25.5 x1010CFU/mL and 13x1010 CFU/mL respectively. The stationary phase on the curve begins at the 24th to the 36th hour. The conclusion of this research is that the red guava juice media can be an excellent growth medium for Lactobacillus sp the conclusion of this research is that the red guava juice media can be an excellent growth medium for Lactobacillus sp.

Keywords: colony number, growth curve, lactobacillus sp, red guava juice

Topic: Biomedical Science