ANTIBACTERIAL EFFECT OF ETHANOL EXTRACTS OF BROCCOLI LEAVES (Brassica oleracea var. italica) AGAINST Propionibacterium acnes IN VITRO
Sayu Putu Yuni Paryati, Henny Juliastuti, Dandi Fery Gunawan

Fakultas Kedokteran Universitas Jenderal achmad Yani

Abstract

Acne vulgaris is an inflammatory disease of the skin that affects 80-100% of people in the world. Acne vulgaris occur because of inflammation by the Propionibacterium acnes. Broccoli leaves (Brassica oleracea var. Italica) have an antibacterial effect. Compounds that have antibacterial effects are flavonoid, saponin, alkaloid, and steroid. This study aims to prove the antibacterial effect of ethanol extract of broccoli leaves against in vitro growth of P. acnes. This study was laboratory experimental. Objects of this study were P. acnes ATCC 11827 and ethanol extract of broccoli leaves with concentrations of 10%, 30%, 50%, 70%, 90%, and 100%. The positive control in this study was clindamycin 10 microgram/disc and DMSO 4% as negative control. Determination of sample repetitions based on Federer^s formula is 4 times. Antibacterial activity test was measured by well diffusion method. The results showed the ethanol extract of broccoli leaves does not have inhibitory power for all concentrations and it was comparable to the negative control of 0 mm. Factors that affect the results in this study could be from temperature and storage time, light exposure, and levels of active compounds in extracts. The sensitivity of P. acnes to the ethanol extract of broccoli leaves could affect the results. The other factor is technical factors such as the number of bacteria inoculated, media selection, and antibiotic carryover. Based on the results of this study, it can be concluded that the ethanol extract of broccoli leaves does not have an in vitro antibacterial effect on P. acnes growth.

Keywords: Antibacterial, broccoli leaves, inhibition, Propionibacterium acnes

Topic: Biomedical Science