Qualitative Testing of Lignocellulolytic Enzyme Production in Fungi Isolated from Sugarcane Filter Cake Waste
Rahmad1, Laode Asrul2, Tutik Kuswinanti2*, Yunus Musa2, Nurmiaty1

1 Departement of Estate Crops Cultivation, Pangkep State Polytechnic of Agriculture, South Sulawesi, Indonesia
2 Faculty of Agricultural Science, Hasanuddin University, Makassar, Indonesia
*Corresponding author: koeswinanti[at]yahoo.com

Abstract

Filter cake is a solid waste extracted from sugarcane roomie containing cellulose, hemicellulose, and lignin. The aim of this study is to examine the ability of fungal isolates found in filter cake waste to produce lignocellulolytic enzymes. Fungal isolates in filter cake samples are isolated, purified, and tested for their ability to produce cellulose and hemicellulase enzymes using Cpazek media (difco). Clear zone testing is carried out by giving iodine solution while ligninase enzyme testing is done using Malt Extract Agar (MEA) media. The isolation results 14 fungal isolates. The highest amount of isolates found includes 4 isolates of Trichoderma sp, 5 isolates of Aspergillus sp, 2 isolates of Penicillium sp, 2 isolates of Fusarium sp, and 1 isolate of Pleurotus sp. As many as 5 isolates produce cellulase enzymes, 5 isolates produce hemicellulase enzyme, and 3 isolates produce lingninase enzyme. Fungal isolates of Aspergillus sp7 produce the highest cellulase enzyme, fungal isolates of Aspergillus sp1 produce the highest hemicellulase enzyme, and fungal isolates of Pleurotus sp produce the highest ligninase enzyme. The results show that there are fungus isolates from filter cake waste that has the high ability to produce lignocellulolytic enzymes.

Keywords: Filter cake sugarcane, isolation, qualitative, fungi, lignocellulolytic enzymes

Topic: Biodiversity and Climate Change