Utilization of tofu wastewater and sugar industry by-products as a medium for the production of antifungal metabolites by Paecylomyces marquandii TP4
Desak Gede Sri Andayani, Desak Gede Tirta Andini

#1 Research Unit for Clean Technology, Indonesian Institute of Sciences, Jl Cisitu, Bandung 40135, Indonesia.
#2 Faculty of Science and Technology, Airlangga University, Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya,60115, Indonesia

Abstract

Paecilomyces marquandii strain TP4 is a saprophytic fungi that isolated from soil taken from Tangkuban Perahu Mountain at West Java Province, Indonesia. The aim of this research is to identify the growth profile of P. marquandii strain TP4 and the antifungal activity against Fusarium sp. Antifungal compound is produced by liquid fermentation method using tofu wastewater and molasses media. The fermentation broth is extracted by liquid-liquid extraction using n-hexane, ethyl acetate and methanol solvents. The result shows that the optimum activity is achieved after 120 hours fermentation. The fermentation broth has antifungal activity against Fusarium sp. where inhibitory diameter was 20 mm. The results of total flavonoid content and total phenolic content of freeze dry fermentation culture were 0.4839 mg/g and 1.171 mg/g, respectively. Ethyl acetate extract shows the highest antifungal activity with MIC 31.25 ppm and MFC 125 ppm.. The FTIR spectrum shows the functional groups of OH, aliphatic CH, C-C, aromatic C=C, CH and C-O. Characterization of antifungal compound based on phytochemical test and FTIR determine to be phenolic group.

Keywords: tofu wastewater, molasses Paecilomyces marquandii Strain TP4, antifungal activity, Fusarium sp., Fermentation

Topic: Environmental Science, Technology, and Education