The Effectiveness of Snail Seromucous and Chitosan as Biological Response Modifiers Immunostimulatory on Lymphocyte Proliferation Agnes Sri Harti, Rahajeng Putriningrum, Nony Puspawati, Yusup Subagio Sutanto
Kusuma Husada University of Surakarta, Indonesia
Abstract
Seromucous snail contains bioactive compounds as glycans, peptides, glycopeptides, and chondroitin sulfate. Chitosan as a result of chitin derivatization has some hydroxyl groups and amine groups which can act as antimicrobials. The substance that can stimulate the immune system is called biological response modifiers (BRM). The aim of this study was to determine the effectiveness of bioactive compounds of snail seromucous and chitosan as biological response modifiers on lymphocyte proliferation. The research method is based on an experimental laboratory. The research methods included isolation seromucous of a snail and the synergistic effect of bioactive compounds of snail seromucous and chitosan as in vitro BRM against lymphocyte proliferation using the MTT reduction method in vitro. Statistical analysis using SPSS 18 version software. There is a synergistic effect of the bioactive compound of snail seromucous 65ug/mL- chitosan 65 ug/mL and the combination ratio of 1: 1 to the optimum lymphocyte proliferation. The potential of snail seromucous and chitosan as effective BRM immunostimulatory and candidates for anti-inflammatory drugs.
