IN VITRO ANTIBACTERIAL ACTIVITY TEST OF 4-(3-(4-CHLOROPHENYL)-1-PHENYL-4,5-DIHYDRO-1H-PYRAZOL-5-YL)-2-METHOXYPHENOL
Estin Nofiyanti (1*), Noer Laelly BTAG (2), Novi Nurjanah (1), Nurcholis Salman (1), Tutik Dwi Wahyuningsih (3)

1) Environmental Engineering Department, Universitas Muhammadiyah Tasikmalaya, 46196 Indonesia

2) Electronical Engineering Department, Universitas Muhammadiyah Tasikmalaya, 46196 Indonesia

3) Chemical Organic Laboratory, Chemistry Department, Universitas Gadjah Mada, Yogyakarta 55281 Indonesia

*estin.nofi[at]umtas.ac.id

Abstract

Synthesis of 4-(3-(4-chlorophenyl)-1-phenyl-4,5-dihydro-1H-pyrazol-5-yl)-2-methoxyphenol as antibacterial agent was performed by cycloaddition reaction. These compound was performed by refluxing the chalcone with phenylhydrazine in acidic condition for 4 h. The structure of the synthesized compound was confirmed by FTIR and 1H-NMR spectrometers. The pyrazoline was screened for antibacterial activity by agar well-diffusion against Staphylococcus aureus, Bacillus cereus, Bacillus subtillis, Escherichia coli and Shigella flexnerri. The pyrazoline was found to exhibits an antibacterial activity and its zone of inhibition/concentration against Staphylococcus aureus (4.75/100), Bacillus cereus (2.50/500), Bacillus subtillis (4.50/300), Escherichia coli (4.75/500) and Shigella flexnerri (3.75/300). The result showed that the pyrazoline which were subtituted with chloro, hydoxy, and methoxy, was able to inhibit the growth of the tested pathogenic bacteria.

Keywords: antibacterial, chalcone, pyrazoline, vanillin

Topic: Physics, Biology, and Chemistry