Question from Mr. Alfian Syarifuddin
2020.11.18 13:43:17

The bacterial suspensions incubated for 3-5 hours were the tested bacteria, namely
SA and Ec. the goal is to maintain the log phase in the test bacteria, so that when
used for testing, the activity to inhibit bacterial growth has interpreted the actual
activity not because the test bacteria died by itself or not from the test sample.

For optimization, the bacteria isolate J4 can produce antibacterial. So, the
incubation time is different, I use 1 to 14 days for optimization.