EFFECTIVENESS OF QUERCETIN FROM KENIKIR LEAVES (Cosmos Caudatus Kunth) IN NANOEMULSION FORMULA AS AN ANTIOXIDANT USING RICE BRAN OIL Siti Hajir, Ratri Ariatmi Nugrahani, Ismiyati, Nurul Hidayati Fithriyah
Master Program of Chemical Engineering, Faculty of Engineering, Universitas Muhammadiyah Jakarta
Abstract
This study was an effort to obtain high yield of quercetin extraction from Kenikir leaves employing several extraction methods, which were maceration, percolation, soxhlation, and reflux, using methanol as solvent followed by quercetin incorporation into a nanoemulsion system with Rice bran oil (RBO) as the oil phase. The resulting nanoemulsion was tested with antioxidant activity analyses using DPPH (1,1-diphenyl-2-picrylhydrazil) reagent. The nanoemulsion preparation was produced from Kenikir leaf powder at 20% concentration. The characterizations of formula consisted of organoleptic tests, analyses of pH, particle size, emulsion type, and determination of quercetin content absorbed in the nanoemulsion system, as well as antioxidant activity testing. The results showed that the highest quercetin yield of 14.26 ppm was produced from the soxhlation method for two hours. The nanoemulsion preparation with 20% Kenikir leaf extract and RBO oil phase was good and stable. The formulations also exhibited 6.3 pH, O/W emulsion type, and 176.8 nm particle size. The absorption of quercetin in the nanoemulsion showed an %EE value of 99.99%, indicating the formulation^s high capacity to absorb quercetin. The antioxidant activity testing using DPPH assay produced an IC50 value of 106.805 ppm.
